human fibroblast cell lines  (ATCC)

Journal: Neuro-Oncology

Article Title: MBD3 deficiency decommissions the nucleosome remodeling and deacetylase complex and orchestrates the epigenetic regulation of gene expression to suppress neuroblastoma progression

doi: 10.1093/neuonc/noaf297

Figure Lengend Snippet: MBD3 knockdown induces cell growth inhibition and apoptosis in human neuroblastoma cell lines. (A-C) Data used for plotting are from DepMap database ( https://depmap.org ). The mRNA expression levels (A, n = 33) and gene effect scores (B, n = 37) of MBD2 and MBD3 across a panel of neuroblastoma cell lines were plotted. Values are log2(TPM + 1) (A) or gene effect scores (B). For (C), the gene effect scores of MBD3 across a panel of cancer cell lines ( n = 1150) derived from different tissues were plotted. The number of cell lines analyzed for each tissue is noted in parentheses after the tissue name. Boxes indicate the median (horizontal line), 25th percentile and 75th percentile; Whiskers indicate the minimum to the maximum (A, B), or distances from the largest and smallest value to each end of the box that are within 1.5× box length (C). Dashed line indicates mean value of the gene effect scores (C). Values were compared with the 2-tailed unpaired t -test. *** P < .001, **** P < .0001. TPM, transcripts per kilobase of exon per million mapped reads. (D-I) Neuroblastoma cell lines (IMR32, SK-N-DZ, SH-SY5Y, and SK-N-SH) and a foreskin fibroblast cell line HFF-1 engineered with a doxycycline-inducible shRNA knockdown system were untreated or treated with 100 ng/mL doxycycline for 4 days. sh Luc was used as a control. The expression of MBD3 was detected by western blot analysis; β-actin was used as a loading control (D). Relative cell growth (E-I) at days 2, 4, and 6 was evaluated, and the color scheme is shown in (I). Values are means ± SEM of triplicate experiments. Mean values were compared with 2-way ANOVA by Dunnett's test. **** P < .0001. (J) Colony formation assay in neuroblastoma cells with a doxycycline-inducible shRNA. Cells were treated with 100 ng/mL doxycycline for 7 days and stained with 0.05% crystal violet. sh Luc was used as a control. (K, L) Neuroblastoma cells with a doxycycline-inducible shRNA were treated with 100 ng/mL doxycycline for 4 days and subjected to cell cycle and apoptosis analysis by flow cytometry. The percentages of cells in G0/G1, S, and G2/M phase of the cell cycle were analyzed with propidium iodide (PI) staining (K). Apoptotic cells were determined by the percentage of Annexin V-positive cells after staining with Annexin V-FITC and PI (L). Values are means ± SEM of 3 independent experiments. Values were compared with the 2-tailed unpaired t -test. * P < .05, ** P < .01, *** P < .001, **** P < .0001, ns, not significant.

Article Snippet: Neuroblastoma cell lines IMR32, SH-SY5Y, SK-N-SH and fibroblast cell line HFF-1 were purchased from National Collection of Authenticated Cell Cultures (NCACC), and SK-N-DZ and SK-N-AS from American Type Culture Collection (ATCC).

Techniques: Knockdown, Inhibition, Expressing, Derivative Assay, shRNA, Control, Western Blot, Colony Assay, Staining, Flow Cytometry

Journal: Biochemistry and Biophysics Reports

Article Title: Preliminary study of cyto-impedance: Molecular profiling of functional impedance in motility-promoting treatment of normal cells

doi: 10.1016/j.bbrep.2026.102476

Figure Lengend Snippet: EH-P002A promotes wound healing in a dose-dependent manner . A. Molecular structure of EH-P002A. B. No detectable cytotoxicity was observed in HFF-1 or Beas-2B cells upon EH-P002A treatment. C. Wound images from mice treated with EH-P002A at two doses, captured at different time points. D. Wound healing rates on Days 7 and 9. Dose-dependent efficacy on Day 7 was observed.

Article Snippet: Human fibroblast cell line HFF-1 (Cat. No. SCRC-1041, ATCC) and bronchial epithelial cell line Beas-2B (Cat. No. CRL-3588, ATCC) were cultured in Dulbecco's Modified Eagle Medium supplemented with 10 % fetal bovine serum in a humidified 5 % CO2 incubator at 37 °C, and confirmed to be free of mycoplasma contamination.

Techniques:

Journal: Biochemistry and Biophysics Reports

Article Title: Preliminary study of cyto-impedance: Molecular profiling of functional impedance in motility-promoting treatment of normal cells

doi: 10.1016/j.bbrep.2026.102476

Figure Lengend Snippet: Dose-dependent cyto-impedance induced by EH-P002A . A . Wound-healing assay images of HFF-1 cells treated with indicated concentrations of EH-P002A, followed by quantification in the right panel. A promoting effect on wound healing was observed at 8 μM, while overdosage of EH-P002A suppressed the migratory ability. B . Images of migrated HFF-1 cells in transwell assays treated with varying EH-P002A concentrations followed by quantification in the right panel, showing promotion at 8 μM and suppression at higher doses. C. The data of wound-healing assay of Beas-2B cells were quantified, showing a promoting effect at 8–20 μM concentrations, and a suppressive effect at a higher concentration of 80 μM. D . Quantification of the transwell assays of Beas-2B cells confirmed dose-dependent cyto-impedance at 40 and 80 μM concentrations. ∗, P < 0.05; ∗∗, P = 0.001; ∗∗∗, P < 0.001.

Article Snippet: Human fibroblast cell line HFF-1 (Cat. No. SCRC-1041, ATCC) and bronchial epithelial cell line Beas-2B (Cat. No. CRL-3588, ATCC) were cultured in Dulbecco's Modified Eagle Medium supplemented with 10 % fetal bovine serum in a humidified 5 % CO2 incubator at 37 °C, and confirmed to be free of mycoplasma contamination.

Techniques: Wound Healing Assay, Concentration Assay

Journal: Biochemistry and Biophysics Reports

Article Title: Preliminary study of cyto-impedance: Molecular profiling of functional impedance in motility-promoting treatment of normal cells

doi: 10.1016/j.bbrep.2026.102476

Figure Lengend Snippet: Differentially expressed genes in cyto-impedance (6 downregulated, 20 upregulated) . HFF-1 cells were treated with EH-P002 for 48 h. The cellular mRNA levels were assessed via whole-genome expression profiling. A. The downregulated genes during cyto-impedance. B. The upregulated genes associated with cyto-impedance.

Article Snippet: Human fibroblast cell line HFF-1 (Cat. No. SCRC-1041, ATCC) and bronchial epithelial cell line Beas-2B (Cat. No. CRL-3588, ATCC) were cultured in Dulbecco's Modified Eagle Medium supplemented with 10 % fetal bovine serum in a humidified 5 % CO2 incubator at 37 °C, and confirmed to be free of mycoplasma contamination.

Techniques: Expressing

Journal: Macromolecular Bioscience

Article Title: Lignin Nanoparticles Containing Cobalt‐Cyanine Complexes: Potential Multifunctional Platforms for Photoacoustic Imaging and Photothermal Treatment of Bacterial Biofilms in Chronic Wounds

doi: 10.1002/mabi.202500532

Figure Lengend Snippet: Cell compatibility of CoPc‐Lig NPs. A) Cell viability of fibroblasts (black bars) and keratinocytes (white bars) exposed to different concentrations of CoPc‐Lig NPs for 24 h. B) Confocal images showing cell internalization of CoPc‐Lig NPs (0.5 mg/mL) by fibroblasts and keratinocytes. The dark areas in brightfield images represent CoPc‐Lig NPs, while the fluorescence channels show cell nuclei (Blue), and cell cytoskeleton (green).

Article Snippet: Bacterial strains ( Staphylococcus aureus ATCC 6538 and Pseudomonas aeruginosa ATCC 9027) and human fibroblast cell line (ATCC‐SCRC‐1041, HFF‐1) were purchased from the American Type Culture Collection (ATCC LGC Standards, Italy).

Techniques: Fluorescence